サワダ　タダシ   澤田　規    所属   保健医療学部 柔道整復学科    職種   教授
言語種別	英語
発行・発表の年月	2019/11
形態種別	研究論文（学術雑誌）
査読	査読あり
標題	Deuterium oxide protects against myocardial injury induced by ischemia and reperfusion in rats
執筆形態	共著
掲載誌名	SCANDINAVIAN CARDIOVASCULAR JOURNAL 2019, VOL. 53, NO. 6,
掲載区分	国内
巻・号・頁	pp.329-336
著者・共著者	Yuko Ishikawa, Hirotoshi Kitagawa, Tadashi Sawada, Tomoyoshi Seto, Kan Takahashi and Toji Yamazaki
概要	Objectives. Although deuterium oxide (D2O) has preservative property on the extracted organ, whether D2O also protects the in situ myocardial injury remains unknown. Using cardiac microdialysis, local administration of D2O through dialysis probe was applied in situ rat heart. We examined the effect of the D2O on the myocardial injury induced ischemia, reperfusion, and chemical hypoxia. Methodology. We measured dialysate myoglobin levels during 30 min of coronary occlusion and reperfusion in the absence and presence of D2O. Furthermore, to confirm the effect of D2O on NaCN induced myocardial injury, we measured the dialysate myoglobin levels with local perfusion of NaCN in the absence and presence of D2O. Results. The dialysate myoglobin levels increased from 177 ± 45 ng/mL at baseline to 3030 ± 1523 ng/mL during 15–30 min of coronary occlusion and further increased to 8588 ± 1684ng/mL at 0–15 min of reperfusion. The dialysate myoglobin levels with 60 min local perfusion of NaCN increased to 1214 ± 279 ng/mL. D2O attenuated myocardial myoglobin release during 15–30 min of coronary occlusion and 0-30 min of reperfusion and 15–60 min of local perfusion of NaCN. Conclusions. D2O might have a beneficial effect of myocardium against ischemia, reperfusion and chemical hypoxia.